We developed an environmentally friendly biofouling resistant membrane by incorporating d-tyrosine onto a commercial nanofiltration membrane using FAU type zeolite nanoparticles that covalently bound to the membrane surface as carriers for slow release. The d-tyrosine loaded membrane had similar water permeability as the unmodified membrane, but greatly reduced initial cell attachment and strongly inhibited subsequent biofilm formation without inactivating the bacteria. The anti-biofouling capacity could be retained for several days.